Useful information:
IMAPlateTM 5RC96 Presentation 2012
IMAPlate Technology Presentation
IMPlateTM 5RC96 Introduction (Video clip in youtube)
IMAPlateTM 5RC96 Q&A
Applications:
Miniature heterogeneous assay:
rapid miniature Troponin I ELISA (5 µl)
miniature ELISA with low volume (5 µl)
miniature ELISA with macro volume (15 - 25 µl)
Miniature homogeneous assay:
miniature protein quantification: 5 µl Bradford assay
miniature protein quantification: BCA assay with sample volume of 2.5 µl
miniature ALK and Lipase assay with macro volume (15 - 25 µl)
Miniature spectroscopic analysis with long pathlength
enhancing conventional ELISA sensitivity 1
enhancing conventional ELISA sensitivity 2 (ELISA kit with precoated plate)
enhancing conventional ELISA sensitivity 3 (ELISA reagent kit)
UV spectrophotometric DNA qantification (touch-loading and pipetting protocol)
UV spectrophotometric DNA qantification (5µl and 15µl pipetting protocol)
UV spectrophotometric Protein qantification (0.5µl and 5µl pipetting protocol)
Miniature cell culture
preparation of spheroids for U87, LN319 and HEK cells
illistrations of the preparation of spheroids and embryoid body
illustrations of 3D cell-based assay
Publications:
1. Spies P, Chen GJ, Gygax D*. Establishment of a miniaturized enzyme-linked immunosorbent assay for human transferrin quantification using an intelligent multifunctional analytical plate Analytical Biochemistry, Volume 382, Issue 1, 1 November 2008, Pages 35-39
2. Peter Spies, Regina Mueller, Guo Jun Chen, Daniel Gygax*. A simple approach to improve the sensitivity of enzyme-linked immunosorbent assay: Using the IMAPlate 5RC96 for result readout Analytical Biochemistry, Volume 397, Issue 1, 1 February 2010, Pages 48-52
3. M. A. Sciotti, G. J. Chen, D. Gygax*. IMAPlate Based Miniature, High Sensitive, Rapid Screening Method for Detecting Bioengineered, Secreted Lipase Activities in Yeast Expression Systems. CHIMIA,Volume 64, No. 11, November 2010, Pages 789-792
4. Theo Sturm et al. Mouse urinary peptides provide a molecular basis for genotype discrimination by nasal sensory neurons. NATURE COMMUNICATIONS | 4:1616 | DOI: 10.1038/ncomms2610 |www.nature.com/naturecommunications
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